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Multiplexed single molecule RNA visualization with a two-probe proximity ligation system
Multiplexed single molecule RNA visualization with a two-probe proximity ligation system
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机译:用双探针接近连接系统多重分子RNA可视化
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摘要
SNAIL provides cost-efficient detection of specific nucleic acids in single cells, and may be combined with flow cytometry to simultaneously analyze large numbers of cells for a plurality of nucleic acids, e.g. at least one, to up to 5, up to 10, up to 15, up to 20 or more transcripts can be simultaneously analyzed, at a rate of up to about 50, 100, 250, 500 or more cells/second. The methods require only two primers for amplification, and may further include a detection primer.
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