METHOD FOR GENERATING NEW MUTATIONS IN ORGANISMS, AND APPLICATION THEREOF

摘要

Provided is a method for creating fixed point mutations in organisms without an artificial DNA template, and an application thereof. The method comprises the following steps: at a specific position in the genome of an organism, two or more DNA breaks are produced in sequential order and respectively self-repaired, a subsequent DNA break being produced on the basis of a new sequence produced after the previous DNA break is repaired.