CRISPR-MEDIATED IDENTIFICATION OF BIOTINYLATED PROTEINS AND CHROMATIN REGIONS

摘要

Aspects of the present invention relate to method and systems which provide extended capability, compared to the existing techniques, for studying the important genome-proteome interactions and chromatin folding and dynamics in higher eukaryotes. Particularly, but not exclusively, the present invention relates to a system involving the CRISPR/ Cas gene editing technology in combination with one or more protein identification techniques based on proximity biotinylation. Certain embodiments involve the use of quantitative mass spectrometry, and/ or next-generation sequencing.