MATERIALS AND METHODS FOR MIXED MODE, ANION EXCHANGE REVERSED PHASE LIQUID CHROMATOGRAPHY

摘要

In various aspects, the present disclosure pertains to high purity chromatographic materials that comprise a chromatographic surface wherein the chromatographic surface comprises a hydrophobic modifier and an ionizable modifier comprising one or more anion exchange moieties that are positively charged when ionized, as well as devices containing such materials. In other aspects, the present disclosure provides methods for mixed mode, anion exchange reversed phase liquid chromatography comprising: (a) loading a sample comprising a plurality of acidic analytes (e.g., acidic glycans) onto a chromatographic separation device comprising such a high purity chromatographic material and (b) eluting adsorbed acidic analytes from the high purity chromatographic material with a mobile phase comprising water, organic solvent, and an organic acid salt, wherein during the course of elution a pH of the mobile phase, an ionic strength of the mobile phase, and a concentration of the organic solvent are altered over time.