Flavin adenine dinucleotide-dependent formate oxidase mutants, nucleic acid molecules, enzyme electrodes, biobattery, and biosensors

摘要

To provide an FAD dependent formic acid oxidase variant which is modified such that formic acid dehydrogenase activity is imparted, a nucleic acid molecule coding a variant, an enzyme electrode using a variant as an electrode catalyst, a bio battery and a biosensor.SOLUTION: An FAD dependent formic acid oxidase variant, a nucleic acid molecule coding a variant, an enzyme electrode utilizing a variant, a bio battery, and a biosensor have an amino acid sequence having at least one of substitution selected from substitution of amino acid residue arginine at a position corresponding to position 60 of specific amino acid sequence to alanine, substitution of amino acid residue asparagine at a position corresponding to position 61 to alanine or glycine, substitution of amino acid residue tyrosine at a position corresponding to position 99 to alanine or glycine, in an amino acid sequence of wild-type flavin adenine dinucleotide (FAD) dependent formic acid oxidase, and have formic acid dehydrogenase activity.SELECTED DRAWING: Figure 3