A Pt-nanoparticle/carbon nanotube modified graphite electrode immobilized with cholesterol oxidase/sol-gel layer was developed for monitoring cholesterol. Using this electrode, cholesterol concentration (4.0×10-6 to 1.0×10-4 mol/L) could be determined accurately in the presence of ascorbic or uric acid, and the response time was rapid (< 20 s). This biosensor has high sensitivity and selectivity.
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