LOW MOLECULAR WEIGHT CHONDROITIN SULFATE, COMPOSITION CONTAINING SAME, AND PREPARATION METHOD THEREFOR AND USE THEREOF

摘要

Disclosed are a low molecular weight chondroitin sulfate and a preparation method therefor. The method comprises: using a macromolecular chondroitin sulfate as a raw material, and subjecting same to production processes of chondroitin sulfate lyase degradation, deproteinization, filtration, sterilization, drying, etc., so as to obtain a low molecular weight chondroitin sulfate with an average molecular weight less than 1000 daltons. The chondroitin sulfate has a narrow molecular weight distribution range, wherein chondroitin sulfate disaccharides account for 43-60%; chondroitin sulfate tetrasaccharides account for 30-45%; the sum of the content of chondroitin sulfate disaccharides and chondroitin sulfate tetrasaccharides is greater than 87%; the total content of oligosaccharides in the low molecular weight chondroitin sulfate is at least 97%; and the content of protein does not exceed 0.5%. Compared with common commercially available macromolecular chondroitin sulfate, the product has a more obvious repair effect on chondrocytes damaged by 1 mM hydrogen peroxide within the concentration of 50-100 μg/mL. The product has a strong repair ability with a repair rate between 14% and 23%, and can be used to treat joint injuries and is an important raw material for medical products, health care products, cosmetics, foods, etc.