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DE-NOVO K-MER ASSOCIATIONS BETWEEN MOLECULAR STATES
DE-NOVO K-MER ASSOCIATIONS BETWEEN MOLECULAR STATES
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机译:分子状态之间的De-novo k-mer关联
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摘要
Provided are methods for preparation and analysis of nucleic acids. Some embodiments include reverse transcribing the RNA with barcoded primers to produce cDNA while maintaining the DNA in the sample, sequencing the DNA and cDNA together, and differentiating the sequenced DNA and cDNA using the barcode or barcodes of the primers. Some embodiments include analyzing the DNA and cDNA sequences of multiple samples separating reads into k-mers, and comparing the k-mers between samples to identify differential sequences between the sequences of the samples.
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