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BETA-GALACTOSIDASE VARIANT HAVING HIGH TRANSGLYCOSYLATION ACTIVITY, AND USE THEREOF

机译:β-半乳糖苷酶变体具有高胰糖基化活性,及其用途

摘要

The present invention relates to a beta-galactosidase mutant having high transglycosylation activity and containing a C-terminal deletion, and the use thereof. More specifically, the present invention relates to a beta-galactosidase mutant having a C-terminal deletion, a gene encoding the same, a recombinant vector containing the gene, a recombinant microorganism transformed with the gene or the recombinant vector, and a method for producing the beta-galactosidase mutant using the recombinant microorganism. Using the high transglycosylation activity of the beta-galactosidase mutant containing the C-terminal deletion according to the present invention, galactooligosaccharide can be efficiently produced in large amounts.
机译:本发明涉及具有高胰糖基化活性并含有C末端缺失的β-半乳糖苷酶突变体,以及其用途。更具体地,本发明涉及具有C-末端缺失的β-半乳糖苷酶突变体,其编码的基因,含有基因的重组载体,与基因或重组载体转化的重组微生物,以及生产方法使用重组微生物的β-半乳糖苷酶突变体。使用根据本发明的含有C-末端缺失的β-半乳糖苷酶突变体的高转基糖基化活性,可以大量有效地生产吡脱硫糖苷。

著录项

  • 公开/公告号EP3305895B1

    专利类型

  • 公开/公告日2021-04-28

    原文格式PDF

  • 申请/专利权人

    申请/专利号EP20160800253

  • 申请日2016-05-19

  • 分类号C12N9/38;C12N15/75;C12N15/80;C12N15/81;C12P19/04;

  • 国家 EP

  • 入库时间 2022-08-24 18:25:06

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