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SUPERNATANT FROM A COCULTURE OF MACROPHAGES AND IRRADIATED LEUKOCYTES, FOR CONTROLLING TUMOUR PROGRESSION OR RESTORING ANTI-TUMOR IMMUNITY
SUPERNATANT FROM A COCULTURE OF MACROPHAGES AND IRRADIATED LEUKOCYTES, FOR CONTROLLING TUMOUR PROGRESSION OR RESTORING ANTI-TUMOR IMMUNITY
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机译:来自巨噬细胞的共培养和辐照白细胞的上清液,用于控制肿瘤进展或恢复抗肿瘤免疫力
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摘要
The invention relates to a method for the resolution of pro-tumour inflammation by means of a pharmaceutical preparation. Leukocytes are isolated from the buffy coat obtained from whole blood and a portion of the leukocytes is placed in a first pouch receiving a differentiation factor and stored in conditions that preserve cellular viability for several days, before a defined, fresh culture medium is supplied, after which said leukocyte portion is left to rest for a further several days, which results in the production of macrophages. A further portion of the leukocytes is placed in a second pouch and irradiated therein. The two pouches are then mixed and the supernatant is recovered to serve as pharmaceutical preparation. The invention also relates to the use in the treatment of cancers, either alone or in combination with other therapies, in human and veterinary medicine.
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