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Penicillin expandase mutants, DNA coding the mutants, reagent kit containing the mutants and the application
Penicillin expandase mutants, DNA coding the mutants, reagent kit containing the mutants and the application
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机译:青霉素扩张酶突变体,DNA编码突变体,含有突变体和施用的试剂盒
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摘要
Provided herein are penicillin expandase mutants, DNA coding the mutants, reagent kit containing the mutants and the application. The penicillin expandase mutants using SEQ ID NO.: 2 in the Sequence Listing as a reference sequence, have at least one amino acid mutation at residue positions corresponding to threonine at position 42 and glutamine at position 126, wherein, amino acid at position 42 is substituted by any other natural amino acid except threonine, amino acid at position 126 is substituted by any other natural amino acid except glutamine. The penicillin expandase mutants of present invention have increased its thermostability and catalytic activity; it is more suitable for commercial and industrial applications.
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机译:本文提供了青霉素扩张酶突变体,DNA编码突变体,含有突变体的试剂盒和应用。青霉素扩张酶突变体使用SEQ ID NO.:2在序列列表中作为参考序列,在对应于位于位置42的苏氨酸的残基位置处具有至少一个氨基酸突变,在位置126处,其中,在第42位的氨基酸是被任何其他天然氨基酸代替苏氨酸,位置126处的氨基酸被除谷氨酰胺外的任何其他天然氨基酸取代。本发明的青霉素扩张酶突变体增加了其热稳定性和催化活性;它更适合商业和工业应用。
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