IMPROVED MEANS AND METHODS FOR INCREASING THE YIELD OF ACETYL-COA FROM GLUCOSE

摘要

Described is a recombinant microorganism characterized by: a) having an inactivation of the gene(s) encoding phosphofructokinase or showing a reduced phosphofructokinase activity as compared to a non-modified microorganism; b) having an inactivation of the gene(s) encoding glucose-6-phosphate dehydrogenase or showing a reduced glucose-6-phosphate dehydrogenase activity as compared to a non-modified microorganism; and c) having a genetic modification to have an increased expression of the gene(s) encoding deoxy-D-ribose-5-phosphate aldolase (EC 4.1.2.4) and/or an increased deoxy-D-ribose-5-phosphate aldolase (EC 4.1.2.4) activity as compared to a non-modified microorganism. Moreover, described is the use of this recombinant microorganism for the conversion of acetyl-CoA into acetone, isobutene, propene and/or isopropanol. Further, described is a method for producing acetone, isobutene, propene and/or isopropanol from acetyl-CoA comprising the steps of culturing the recombinant microorganism of the present invention in a suitable medium.