Exosome active formulation for inhibiting endothelial cell migration, and preparation method and application

摘要

An exosome active formulation for inhibiting endothelial cell migration, and a preparation method and use thereof include the following steps: isolating primary umbilical vein endothelial cells and performing cell culture and passage; adding anisodamine to a culture medium of the subcultured endothelial cells to pretreat the endothelial cells, and then replacing the culture medium with a new endothelial cell culture medium and adding TNF-α to continue the culture of the endothelial cells; extracting exosomes from the TNF-α containing endothelial cell culture medium obtained after the endothelial cells are cultivated; and identifying the exosomes. The exosome active formulation for inhibiting endothelial cell migration prepared by the preparation method of this disclosure and its use in the antitumor drugs.