CELL-CELL INTERACTION ANALYSIS VIA DROPLET MICROFLUIDICS

摘要

The present invention provides systems, kits, and methods for analyzing cell-cell interactions, such as transmembrane proteins binding to surface displayed variable regions, via discrete entity (e.g., droplet) microfluidics. In certain embodiments, a plurality of first discrete entities and a plurality of second discrete entities are merged on a substrate to generate a plurality of merged fixed entities (e.g., fixed via an electrical force), each of which contains one cell expressing a transmembrane (TM) protein and labeled clonal cells displaying a heterologous antibody variable region. In certain embodiments, any binding of the clonal cells to the TM expressing cell is detected in each merged fixed entity, and the clonal cells found to bind are treated in order to sequence the nucleic acid encoding the variable region.