A Method for De novo Root Regeneration of Plant based on an Ultradian Rhythm

摘要

The super week rhythm (UR) circulates in a cycle of less than 24 hours. However, the control and function of this rhythm is not clear. The present inventors confirmed a circadian-independent UR for a period of ~3 hours in the resected Arabidopsis leaves. Gene expression and functional analysis suggests that UR is a positive regulator of denovo root regeneration (DNRR). 4,073 genes including phytohormone- and DNRR-linked genes representing UR were identified by transcriptomic analysis. The present inventors identified rou (regulator of ultradian rhythm) variants using a forward genetic screen, and confirmed that EAR1 (Enhancer of ABA co-Receptor 1) is essential for UR cycling. rou1The variant showed a lack of UR and reduced root regeneration and related gene expression. Hormonal treatment altered EAR1 levels in the transcriptional and post-translational stages, indicating that EAR1 mediates dynamic hormone-related gene expression in resected leaves, producing UR and promoting DNRR. According to the present invention, it can be confirmed that leaf ablation induces URs and leads to DNRR through hormonal regulation, which is a possible survival mechanism in plants.