Pathological specimen, method for producing pathological specimen, and method for acquiring fluorescence image

摘要

A signal of fluorescence emitted from a fluorescent particle of a pathological specimen can be increased in sensitivity and be stabilized, thereby resulting in an enhancement in retrieval accuracy of information from a fluorescence image. A pathological specimen including a tissue section subjected to a treatment (immunostaining/FISH staining treatment) for fluorescence-labeling of an objective biomaterial with a fluorescent particle observable in a dark field, based on an immunostaining or FISH method; a packed layer with which the tissue section is covered; and a protection layer with which the packed layer is covered; whereinthe refractive indexes of the fluorescent particle, the packed layer and the protection layer (measurement wavelength: 589 nm; measurement temperature: 20° C.; in all) satisfy the conditions of Expressions (1) and (2):|n1−n2|≤0.20  Expression (1)|n2−n3|≤0.15  Expression (2)n1: refractive index (fluorescent particle)n2: refractive index (packed layer)n3: refractive index (protection layer).