PATHOLOGICAL SPECIMEN, METHOD FOR PRODUCING PATHOLOGICAL SPECIMEN, AND METHOD FOR ACQUIRING FLUORESCENCE IMAGE

摘要

A signal of fluorescence emitted from a fluorescent particle of a pathological specimen can be increased in sensitivity and be stabilized, thereby resulting in an enhancement in retrieval accuracy of information from a fluorescence image. A pathological specimen including a tissue section subjected to a treatment (immunostaining/FISH staining treatment) for fluorescence-labeling of an objective biomaterial with a fluorescent particle observable in a dark field, based on an immunostaining or FISH method; a packed layer with which the tissue section is covered; and a protection layer with which the packed layer is covered; whereinthe refractive indexes of the fluorescent particle, the packed layer and the protection layer (measurement wavelength: 589 nm; measurement temperature: 20°C; in all) satisfy the conditions of Expressions (1) and (2): $\left|\mathrm{n}1-\mathrm{n}2\right|\le 0.20\mathop{\mathrm{image}}$$\left|\mathrm{n}2-\mathrm{n}3\right|\le 0.15\mathop{\mathrm{image}}$n1: refractive index (fluorescent particle)n2: refractive index (packed layer)n3: refractive index (protection layer).