Primers and probes for detection of sacbrood virus, Paenibacillus larvae and Melissococcus plutonius, and method for detecting sacbrood virus, Paenibacillus larvae and Melissococcus plutonius using the same

摘要

The present invention is a first forward primer described in the nucleotide sequence of SEQ ID NO: 1; A first reverse primer described by the nucleotide sequence of SEQ ID NO: 2; A second forward primer represented by the nucleotide sequence of SEQ ID NO: 4; A second reverse primer represented by the nucleotide sequence of SEQ ID NO: 5; A third forward primer represented by the nucleotide sequence of SEQ ID NO: 7; And it relates to a primer set for detecting cystoid rot, American buzz disease and European buzz disease pathogens consisting of a third reverse primer represented by the nucleotide sequence of SEQ ID NO: 8, wherein the primer and probe set of the present invention includes cystoid rot disease, US buzzer Disease and European buzz disease pathogens can be specifically and simultaneously detected, and has superior sensitivity compared to known primer sets, so it can be usefully used for detection of cystoid rot, American buzz disease, and European buzz disease pathogens, It can be helpful in preparing effective quarantine measures against the above pathogens.