Magnetic bead nucleic acid aptamer multitarget molecule simultaneous detection method and kit

摘要

The invention belongs to the field of molecular biology, and is based on the molecular recognition of molecular target aptamers and nucleic acid aptamers, in combination with magnetic separation and purification can enable the standardization of the detection of multimolecular sets and multifunctional group information through mass spectrometric molecular qualitative and quantitative analysis Demonstrate correlation between the molecular and functional sets of the body or tissue. The invention uses a magnetic separation to easily complete the separation of the target molecule sets, by means of the specificity and high affinity of the aptamer, the target molecule sets are effectively obtained, while using mass spectrometry, the multimolecules can effectively determine qualitative and quantitative, realize the secondary molecular detection and the accuracy improve. The uniformity of the qualitative and quantitative determination of multimolecules can better reflect the interrelationships between molecules and also reveal the interrelationships between bodily functions, which is conducive to proteomics and genome research as well as clinical molecular tests.