AMH-INH-GNIH TRI-EXPRESSION GENE VACCINE OF IMPROVING FECUNDITY OF ANIMALS, PREPARATION METHOD AND APPLICATION

摘要

The invention provides an AMH-INH-GNIH tri-expression gene vaccine capable of improving fecundity of animals and a preparation method of engineering strain thereof. The engineering strain was deposited in China Center for Type Culture Collection on Aug. 15, 2018, with deposit No.: CCTCC NO:M 2018544. When the engineering strain is used for direct immunization of animals or immunization of animals after being mixed with DNA vaccine adjuvant, the fecundity of the animals can be effectively improved. The tri-expression gene is a tri-expression non-resistant DNA plasmid of Mullerian duct resisting hormone, inhibin and gonadotropin release restraining hormone, which can be used for direct immunization of the animals through mucosa immunization to generate antibodies in the manner of being sprayed to noses, orally administered, blended into feeds and the like. Since the gene vaccine does not contain a resistant gene, exogenous antibiotics do not need to be introduced for screening, and antibiotic residues are not generated. Compared with other gene vaccines which need plasmid extraction and purification, high production cost and inconvenient intramuscular injection, and enable the animals to generate stress response, the gene vaccine of the invention is lower in production cost and more convenient to use, and is free from resistance and injection stress response.