METHOD FOR OVERCOMING SELF-INCOMPATIBILITY OF DIPLOID POTATOES

摘要

Disclosed is a method for overcoming self-incompatibility of diploid potatoes, which comprises: (1) selecting a target fragment; (2) constructing a gene-targeting recombinant vector; (3) achieving a loss-of-function mutation of the intracellular S-RNase gene; (4) regenerating a plurality of potato plants; (5) specifically amplifying a DNA segment of the S-RNase gene in a regenerated plant, wherein the DNA segment containing the target fragment; (6) selecting a regenerated plant in which the S-RNase gene is edited; (7) further screening the selected gene-edited plant for a diploid gene-edited plant line; (8) propagating and planting the selected gene-edited plant line, and identifying the self-compatible phenotype at the flowering stage; and (9) sequencing the gene amplification products of the harvested offspring of the self-compatible plant, and detecting the inheritance and isolation of the offspring in which the target gene is edited. The advantage of the present invention lies in that a simple, accurate and efficient method for overcoming the self-incompatibility of diploid potatoes is found.