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METHODS, COMPOSITIONS AND KITS USEFUL FOR PH GRADIENT CATION EXCHANGE CHROMATOGRAPHY
METHODS, COMPOSITIONS AND KITS USEFUL FOR PH GRADIENT CATION EXCHANGE CHROMATOGRAPHY
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机译:对pH梯度阳离子交换色谱有用的方法,组合物和试剂盒
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摘要
The present disclosure relates to methods, compositions and kits useful for the enhanced pH gradient cation exchange chromatography of a variety of analytes. In various aspects, the present disclosure pertains to chromatographic elution buffer solutions that comprise a first buffer salt, a second buffer salt, a third buffer salt, and fourth buffer salt. The first buffer salt may be, for example, a diprotic acid buffer salt, the second buffer salt may be, for example, a divalent buffer salt with two amine groups, the third buffer salt may be, for example, a monovalent buffer salt comprising a single amine group, and the fourth buffer salt may be, for example, a zwitterionic buffer salt. Moreover, the buffer solution has a pH ranging from 3 to 11.
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