VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) FOR BETTER WOUND HEALING THROUGH ENHANCED ANGIOGENESIS

摘要

The present invention discloses and claims a functionally active peptide of VEGF of SEQUENCE ID NO.: 3 through primers for RT-PCR for cloning the c-DNA obtained from VEGF, comprising a forward primer of 28 bp under SEQUENCE ID NO.: 2 and the reverse primer under SEQUENCE ID NO.: 1 of 32 bp. In addition, the present invention discloses a process of preparation of VEGF comprising the steps of isolating the RNA of VEGF (SEQUENCE ID NO.: 3); RT-PCR of VEGF using primers of SEQUENCE ID NO.: 1 and 2, and collecting the purified c DNA; ligating the clones as obtained with the vector pTZ57R/T; preparing the competent cells; transforming the cloned cells after selecting through antibiotic resistance in vector; isolating the protein obtained; and purifying said obtained VEGF protein.