METHOD FOR INDUCING DIFFERENTIATION OF INTERMEDIATE MESODERMAL CELL TO RENAL PROGENITOR CELL, AND METHOD FOR INDUCING DIFFERENTIATION OF PLURIPOTENT STEM CELL TO RENAL PROGENITOR CELL

摘要

A method of producing renal progenitor cells from pluripotent stem cells, the method including the following Steps (i) to (vi): (i) a step of culturing pluripotent stem cells in a medium containing FGF2, BMP4, a GSK-3β inhibitor, and retinoic acid or a derivative thereof; (ii) a step of culturing cells obtained in Step (i) in a medium containing FGF2, a GSK-3β inhibitor, and BMP7; (iii) a step of culturing cells obtained in Step (ii) in a medium containing FGF2, a GSK-3β inhibitor, BMP7, and a TGFβ inhibitor; (iv) a step of culturing cells obtained in Step (iii) in a medium containing FGF2, a GSK-3β inhibitor, BMP7, activin, and a ROCK (Rho-kinase) inhibitor; (v) a step of culturing cells obtained in Step (iv) in a medium containing retinoic acid or a derivative thereof, and FGF9; and (vi) a step of culturing cells obtained in Step (v) in a medium containing a GSK-3β inhibitor and FGF9, to induce renal progenitor cells from intermediate mesodermal cells.