The present invention relates to CO2 capture from gas mixtures by use of gas separation membranes. In particular, the invention relates to a gas separation membrane comprising: a gas permeable or porous support layer; and at least one CO2 selective polymer layer comprising carbonic anhydrase (CA) enzymes fixed within the at least one CO2 selective polymer layer. The present invention also relates to the method of separating CO2 from a gas and to the use of the gas separation membrane.
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