MANUFACTURING METHOD FOR RATIO ELECTROCHEMICAL MIR3123 APTAMER SENSOR BASED ON METAL ORGANIC FRAMEWORK COMPOUND

摘要

A preparation method for a ratio electrochemical miR3123 aptamer sensor based on a copper-based metal organic framework (Cu-MOF) compound doped with black phosphorus nanosheets (BPNSs) and thionine (TH). TH and a Cu-MOF precursor undergo a reaction for preparing TH/Cu-MOF, BPNSs are applied by dropwise coating to prepare BPNSs/TH/Cu-MOF, and the BPNSs/TH/Cu-MOF is applied to an electrode by dropwise coating; ferrocene (Fc)-labeled DNA single-stranded aptamers are adsorbed on the BPNSs to prepare aptamer-BPNSs/TH/Cu-MOF; a target molecule miR3123 is bonded with the DNA single-stranded aptamers Fc-DNA, Fc-DNA is enabled to be separated from the BPNSs, and therefore, Fc electrochemical signals are weakened while TH signals are slightly affected. The ratio electrochemical miR3123 aptamer sensor is constructed by fitting the linear relation of current peak intensity ratio IFc/ITH and miR3123 concentration.