DIRECT MICRORNA SEQUENCING USING ENZYME ASSISTED SEQUENCING

摘要

Provided are a method of determining the sequence of a short RNA, and the seqencing library and system thereof. The method is performed by enzyme assisted sequencing, especially "Nanopore Induced Phase-Shift Sequencing (NIPSS)", using a DNA-RNA chimeric substrate, and can be used to sequence short RNA such as miRNA, siRNA or piRNA.