MULTI-COLOR SYSTEM FOR REAL TIME PCR DETECTION

摘要

The present inventive concept relates to a system for monitoring a PCR-reaction in a microfluidic reactor. The system comprises: a first light source illuminating the microfluidic reactor through a first excitation light filter providing light of a first excitation wavelength range adapted to excite a first fluorophore in the microfluidic reactor, whereby fluorescent light of a first emission wavelength range is emitted by the first fluorophore; a second light source illuminating the microfluidic reactor through a second excitation light filter providing light of a second excitation wavelength range adapted to excite a second fluorophore in the microfluidic reactor, whereby fluorescent light of a second emission wavelength range is emitted by the second fluorophore; a The system further comprises a first emission filter adapted to transmit fluorescent light of the first emission wavelength range and block fluorescent light of the second emission wavelength range; a second emission filter adapted to transmit fluorescent light of the second emission wavelength range and block fluorescent light of the first emission wavelength range. The system additionally comprises first imaging optics adapted to image the microfluidic reactor onto a first imaging surface, by fluorescent light of the first emission wavelength range whereby the image on the first imaging surface is indicative of a first reaction parameter of the PCR-reaction associated with the first fluorophore; and second imaging optics adapted to image the microfluidic reactor onto a second image surface, by fluorescent light of the second emission wavelength range, thereby monitoring a second reaction parameter of the PCR-reaction associated with the second fluorophore.