DIFFERENTIATED TENDON CELLS DERIVED FROM PLURIPOTENT PROGENITOR CELLS AND METHODS OF USE THEREOF

摘要

The present disclosure relates generally to differentiated tendon cells derived from pluripotent stem cells and methods of making and using such cells. In one aspect, provided is a method of generating a population of tenocytes comprising: (a) culturing multipotent mesoderm in serum-free medium in the presence of transforming growth factor P (TGFP) and optionally one or more of fibroblast growth factor (FGF), sonic hedgehog (SHH), smoothened agonist (SAG), Noggin, Activin A, growth differentiation factor 7 (GDF7), CD1530, and LDN-193189 to produce tenocytes, and (b) optionally isolating the tenocytes.