RECOVERY OF HEPATIC FUNCTION OF CULTURED HEPATOCYTES

摘要

Disclosed herein are reagents, methods, compositions, and kits for use in systems for "recovering" from initial de-differentiation resulting from cell culture. In some embodiments, then, this disclosure provides reagents and systems for plating cryopreserved human hepatocytes onto collagen-coated plates to establish a 100% confluent culture and replenish culture medium every two to three days to remove cellular waste products and replace spent nutrients.