A method for the production of in the adsorption equilibrium will be standing depot vaccines

摘要

Depot vaccines comprising an antigen absorbed on a metal oxide are made by mixing (a) an antigen consisting of a virus, a bacterial antigen, a toxin produced by bacteria or an inactivation product of any of these, (b) a finely-divided metal oxide which is non-injurious to human and animal tissue and possesses a molar surface area of about 40,000 to 80,000 square metres, (c) buffering and isotonicizing substances, (d) water, and optionally (e) substances for raising the viscosity of the vaccine for example gelatine. The proportions of the ingredients are so chosen that the mixture contains per ml. about 106 to 109 virus or bacterial particles or the equivalent quantity of toxin or toxoid, and a quantity of metal oxide corresponding to a surface area of about 2 to 20 square metres. The mixture is thoroughly agitated for about 20 to 40 minutes at a temperature of about 0 DEG to 30 DEG C. The vaccines may be cautiously dehydrated, preferably by lyophilization to give stable dry preparations which can be readily reconverted into vaccines by recombination with the previously removal liquid medium and shaking for a short time. Examples given of (a) antigens are: all strains of influenza virus, strains A/Asia/1/57, PR8, B/Berlin/7/57, A1/England/19/55, A1/Dutch/56 being specifically mentioned, mumps virus strain "Enders," poliomyelitis virus, foot and mouth disease virus, viruses of the ECHO group, viruses of the coxsackie group, measles virus; bacterial antigens of B.Salmonella typhi, B.Erysipelas, B.Brucella Abortis Bang., B.Hemophilus Pertusses, B.Tuberculosis; the toxins and toxoids derived from B.Diphtheri Haemopholis, B. Tetani; of (b) metal oxides; those of aluminium, zirconium, magnesium, iron; of (c) additional agents; sodium chloride, primary potassium phosphate, secondary sodium phosphate.