Centrifugation of whole blood to separate eosinophils

摘要

Eosinophil leucocytes are extracted from mammalian blood, e.g of a horse or human, by centrifuging the blood, optionally after initial sedimentation to remove the bulk of the erythrocytes, discarding the serum and "buffy coat layer" of the sediment and separating the eosinophils or an eosinophil-containing material from the remaining heavier fraction of the sediment, e.g. by lysing the accompanying erythrocytes by addition of saline and recentrifuging. The eosinophils obtained may be disrupted, e.g. by use of a homogenizing mill, preferably after freezing and thawing, or by ultra-sonic disruption to give a material which may be freeze-dried and which has antihistaminic and possibly general anti-inflammatory activity.