Method of in vitro synthesis of the nucleic acid, biologically active enzymatic catalysts of this synthesis and new biologically-active products obtained

摘要

1,137,170. Nucleic acids; enzymes. UNIVERSITY OF ILLINOIS FOUNDATION. 6 Sept., 1966 [29 Sept., 1965; 18 March, 1966; 23 June, 1966], No. 39867/66. Heading C3H. Biologically active intact nucleic acid is synthesized in vitro by providing an activated system capable of synthesizing biologically active nucleic acid for prolonged or extensive periods, which system includes biologically active intact nucleic acid, the specific replicase for said nucleic acid which is free of detectable destructive contaminants, and nucleotide base component or components, and recovering the synthesized nucleic acid from said system. The replicase may be obtained from a bacterium (e.g. E. coli) infected with the appropriate nucleic acid or with a virus containing the nucleic acid.