PROCESS FOR THE PREPARATION OF A BASIC WATER-SOLUBLE ANTIBIOTIC BY GROWING A MICRO-ORGANISM OF THE GENDER MICROMONOSPORA UNDER APPROPRIATE CONDITIONS, AS WELL AS A METHOD OF PREPARATION OF A PHARMACEUTICAL ANTIBIOTIC APPLICATION ANTIBIOTIC APPLICATION METHOD TO BRING.

摘要

1,274,518. Antibiotic 66-40. SCHERICO Ltd. 24 June, 1969 [27 June, 1968; 16 Dec., 1968], No. 31887/69. Heading C2A. Novel Antibiotic 66-40 having the formula and characterized as being a basic pseudooligosaccharide having the following properties: elemental analysis, C = 49À80, H = 8À20, N = 14À95 and 0=27À05; molecular weight 447À26; empirical formula C 19 H 37 N 5 O 7 .H 2 O; melting point 185-190‹ C.; equivalent weight 92; pKa 8À0; []SP26‹/SP D + 188À9‹ (C = 0À3% in H 2 O); U.V. absorption: transparent between 220 and 440 mÁ; I.R. absorption peaks at 2À98, 3À05, 3À16, 3À35- 3À50 (mull), 5À93, 6À25, 6À82 (mull), 7À25 (mull), 8À77, 9À00, 9À47, 9À72-10À07, 10À46, 11À97, 12À75 and 13À45-13À90 Á; and forming hydrates and other solvates, condensation products with aldehydes and ketones and salts with acids, is prepared by aerobically cultivating Micromonospora inyoensis (NRRL 3292). The condensation products, which are probably Schiff bases, are formed by reacting 66-40 with aldehydes and/or ketones such as acetaldehyde, acetone, methyl - ethyl - ketone, crotonaldehyde, furfural, cyclopentylacetaldehyde, vanillin, veratraldehyde, benzophenone, benzaldehyde, acetophenone, salicylaldehyde and pyridoxal. Antibiotic 66-40 has bacteriostatic activity on Gram-positive and Gram-negative bacteria and Rickettsia. Pharmaceutical compositions comprise the antibiotic 66-40 or its acid addition salts or aldehyde or ketone condensation products together with a pharmaceutically acceptable carrier or diluent, and may be in forms for topical, oral or parenteral administration.