method and device for simultaneous application of several chromatographic separations on columns, in particular mixtures of aminosaeuren and similar materials

摘要

1,095,569. Chromatography elution and analysis system. CESKOSLOVENSKA AKAD. EMIE VED. April 30, 1965 [May 4, 1964], No. 18295/65. Heading B1X. A plurality of chromatographic separations are carried out simultaneously by supplying eluent to a pump which passes the eluent to a distributor which feeds a plurality of columns cyclically. As illustrated in the Figure, eluent is passed by pump Pv to distributor Kv and thus in sequence via dosers D 1 -D4 to columns V 1 -V4. A further pump PN and distributor KN feed a reagent (e.g. ninhydrin reagent for amino-acid separations) to the eluate streams in sequence, the two distributors preferably being in phase. Three-way cocks d 1 -d4, provide for switching the eluate to drain. The tubes from the columns are of capillary dimensions and pass the eluate-reagent stream to capillary reactors KR 1 - KR4 in a common constant temperature bath. The streams then pass to photometric cells F 1 - F4 having measuring elements E 1 - E4 connected to a common recorder Z. Finally the streams are led to a distributor KF which can conduct any stream to a flow meter B while the remaining streams are discharged to drain. Each measuring cell outlet may be fitted with a flow meter. A known solution may be used in one column, and the recorder may be responsive to a light intensity ratio.