NEW ANTIBIOTIC SUBSTANCE AND PROCESS FOR THE EXTRACTION OF SUCH AN ANTIBIOTIC SUBSTANCE FORM A NEW STRAIN OF PSEUDOMONAS

摘要

1314019 Antibiotics from Pseudomocas Rollandi ALBERT ROLLAND SA 17 July 1970 [19 July 1969] 36428/69 Heading C2A In a process for producing an antibiotic whose infra-red spectrum is shown in Fig. 3, a strain of Pseudomonas Rollandi is aerobically cultured at 12-42‹ C., preferably 28‹ to 37‹ C., in a culture medium for a sufficient period of time to achieve the stationary growth phase of the Pseudomonas, the resulting Pseudomonas is then separated and ground, the resulting ground material is dehydrated, and the dehydrated material is extracted with methanol to give a methanolic solution of the crude antibiotic. The methanolic extract may be evaporated to dryness, extracted with a chlorinated solvent, e.g. chloroform, methylene chloride or trichloroacetic acid, followed by, or preceded by treatment with hexane or heptane to remove lipids. The resultant product may be extracted with ethanol to purify the antibiotic still further. The antibiotic comprises a peptide-ester or a depsipeptide component of average molecular weight 1000 to 2000, which can be isolated as slightly beige crystals, m.p. 155-156‹ C., D (measured at 20‹ C. in ethanol) = - 46 degrees and having the infra-red spectrum shown in Fig. 3 of the accompanying drawings and a second component which is a mixture or complex of dipeptide and in part depsi-peptide character, which can be isolated in a semi-crystalline, ninhydrinpositive form, which is soluble in dilute acetic acid and has an Rf of 0À35 on a cellulose plate when using the solvent system butanol-acetic acid-water, 12 : 3 : 5. Pharmaceutical compositions having antibiotic properties contain an antibiotic as described above in conjunction with a pharmaceutically acceptable excipient or carrier.