A method for the production of 9 - beta - d - ribo furanoside - 5 - mono -, - di- and - tri - phosphates of 2 - substituted 6 - hydroxy purines

摘要

1,200,826. Nucleotides by fermentation. KYOWA HAKKO KOGYO CO. Ltd. 1 Oct., 1968 [13 Oct., 1967], No. 46518/68. Heading C2C. 2-Substituted - 6 - hydroxypurine - 9 - # - D - ribofuranoxide - 5SP1/SP - phosphoric and/or pyrophosphoric acid esters of general formula where R is CH 3 , CH 2 -CH 3 , SH, SCH 3 , SCH 2 CH 3 , NHCH 3 or N(CH 3 ) 2 and X is -P(O)(OH) 2 , -P(O)(OH)-O-P(O)(OH) 2 or -P(O)(OH)- O - P(O)(OH) - O - P(O)(OH) 2 are prepared by culturing a suitable microorganism under aerobic conditions at a temperature of 20‹ to 40‹ C. and a pH of 4À0 to 9À5 in an aqueous nutrient medium containing the corresponding 2 - substituted - 6 - hydroxy - purine. Many micro-organisms are disclosed.