Profiling Analysis of Mono-, Di- and Tri-phosphate Nucleotides Using Capillary Ion Chromatography Mass Spectrometry

摘要

A Cap IC-MS/MS method was develop for the targeted profiling quantitation of nucleotides in biological samples. This method was evaluated and demonstrated the following capabilities: (1) Excellent chromatographic separation for target analytes; (2) Selective SRM MS/MS detection capable of differentiating closely eluted analytes; (3) Ultra-sensitive detection of target analytes down to 1 nM with only few μL sample consumption; (4) Successful application for biological sample analysis.