AN AUTOMATED DIRECT METHOD FOR THE DETERMINATION OF INORGANIC PHOSPHATE IN SERUM

摘要

A direct method is provided for the spectrophotometric determination of inorganic phosphate in fluids, particularly body fluids, such as blood serum. The process requires only a single reagent addition and comprises reacting the phosphate-containing fluid with an ammonium molybdate solution and thereafter measuring the absorbance within a specified time interval before the reaction has measurably proceeded and at the end of the reaction by means of a centrifugal analytical photometer. Inasmuch as a linear relationship exists between the phosphate concentration and the change of absorbance, the concentration is an unknown sample can be conveniently calculated by comparison with the results obtained from the simultaneous measurement of a sample of known concentration.