Enzymatic assay of metabolic substrates - by measuring reaction velocity when it has stabilised at low enzyme concn.

摘要

Enzymatic determination fo substrate, esp. metabolite concns., comprises starting the reaction by adding the enzyme then measuring teh reaction velocity (dE/dt), which is characteristic of the instantaneous substrate concn., only after the perturbation caused by introducing the enzyme has decayed and only during the period of time when the velocity at low enzyme concn. is practically constant e.g. 15-30 sec. Partic. for analysing blood glucose but also for blood alcohol, lactic acid or pyruvic acid. Analysis kis very rapid and method can be used where two enzymes are needed for reaction e.g. hexokinase and glucose-6-phosphate dehydrogenase in glucose assay.