PROCESS FOR THE ENZYMATIC DETERMINATION OF GLUCOSE WITH A GLUCOSE-OXIDASE/PEROXIDASE ENZYME SYSTEM

摘要

Process for enzymatic determination of glucose with a glucose-oxidase-peroxidase enzyme system involving the use of a reduced acceptor which is oxidized. As reduced acceptor in the enzymatic reaction catalyzed by peroxidase, a ferrocyanide salt is oxidized to ferricyanide; then a further reducing system is introduced, which system is oxidized by the ferricyanide, with the formation of a colored quinone compound, the reaction between ferricyanide and the reducing system taking place spontaneously.