Discrete plasmid construction from chromosomal genes in pseudomonas

摘要

A transfer plasmid (factor K) is transferred from a first Pseudomonas organism to a second Pseudomonas organism. Factor K induces the mobilization of genes from the chromosome of the second Pseudomonas organism and under proper conditions will transfer the mobilized genes to a recipient organism in which those particular genes have been deleted. The factor K and the mobilized genes enter the recipient and become established there as stable separate plasmids constituting a plasmid aggregrate. Non-transmissible artificial hydrocarbon degradative plasmids have been created in Pseudomonas organisms in this manner and (with factor K present in the host to accomplish the transfer) have been transmitted to other Pseudomonas recipients.