Process for extracting and purifying 3,6-bis-(5-chloro-2- piperidinyl)-2, 5-piperazine dione

摘要

This invention relates to the process for the purification of 3,6- bis(5-chloro-2-piperidinyl)-2,5-piperazinedione dihydrochloride, also known as Compound 593A, obtained as the free base from the fermentation broth of a strain of Streptomyces griseoluteus designated as NRRL 3412 by adsorbtion of the compound on a resin comprising a nonionic macro porous copolymer of styrene cross-linked with divinylbenzene. The resin is eluted with an organic aqueous dilute mineral acid solvent mixture in which the adsorbed compound is soluble. The compound is extracted from the eluate and further purified by fractional crystallization.