Prodn. of live virus vaccine - for immunising chickens against Marek's disease, by incubating in a multiplate appts.

摘要

A large scale tank process for producing a live virus vaccine for immunising chickens against March's disease comprises incubating a mixt. of viral-free cells, growth medium and isolated turkey herpes virus in a multiplate appts. which comprises a plurality of discs mounted on a rotable shaft, the incubation being started before any substantial cell growth has taken place in the appts., continuing the incubation until a satisfactory virus level is obtd. The vaccine for immunising chickens against March's disease is produced at a low cost in the desired titre without any further concn. steps which may result in contamination and increased handling costs.