A procedure for obtaining purified products of protein isolation. (Machine-translation by Google Translate, not legally binding)

摘要

A process for obtaining purified protein isolation products, characterized in that it comprises: l) suspending or in the form of a paste a cake of protein substance containing compounds of mucilaginous consistency, with a volume in liters equal to 10 to 15 times, and preferably from 3 to 5 times, the weight in kg of the cake, of a solution of one or more alkaline earth metal salts, among them magnesium, which also contains na sulfite, and add to the suspension or paste obtained a concentrated alkali solution, in sufficient quantity to allow the solubilization of the proteins, (2) separating, in a known manner, the protein extract of the insoluble residue, 3) acidifying the resulting clarified protein extract with the help of an acid solution to a ph between 4.5 and 5.5 to precipitate the proteins, and 4) to separate from the mother liquor, in a known manner, the protein precipitate formed, by submitting p said protein precipitate after washing. (Machine-translation by Google Translate, not legally binding)