Analysis of blood or breath - using e.g. pyrocatechol and Mohr's salt for oxygen, diluted blood for carbon monoxide and fuchsin and hydrazine hydrate for carbon dioxide

摘要

Determination of O2, CO and CO2 content of gaseous and liq. samples comprises continuous addn. of a constant amt. of (a) 25 mmol/l. pyrocatechol in 0.01N NaOH or 25 mmol/l. pyrogallol in 1N NaOH and e.g., 5 mmol/l. Fe(NH4)2(SO4)2 to increase the colour strength for O2 determination; (b) blood diluted with distilled water in ratio 1:200 or 1:1000 for CO determination; and (c) 0.1-0.2 mmol./l. fuchsin and 2-4 mmol/l. hydrazine hydrate for CO2 determination; and measuring the concn. of the coloured substances by measuring the absorption on a photometer. Alternatively a single determn. may be carried out by leaving the reagents and gas or liq. sample in a cuvette until reaction is complete (ca. 2 mins.) and measuring the change in absorption. Esp. for determining the O2, CO and CO2 content of the breath and of blood, but also of the environment and in fermentation processes etc. The amts. of the three gases can be determined simultaneously and rapidly (reaction takes 2 mins. cf. 5 mins. in previous processes). The reagents are cheap. The appts. is simple, and the appts. for single measurements is easy to move and can be used in field research e.g., in monitoring environmental pollution.