ERYTHROCYTES SENSITIZED WITH A SINGLE IMMUNOGLOBULIN OR COMPLEMENT COMPONENT

摘要

A set of four cell suspensions containing erythrocytes monospecifically sensitized with IgG, IgM, C3, and C4. The suspensions provide control cells to determine the purity and strength of the antibodies to these immunoglobulins or complement components contained in antisera used for clinical testing. Strict control of the pH, temperature, and EDTA concentration of a solution to which blood is added allows for the elective production of C3- or C4- sensitized cells. Selecting a serum with IgG or IgM antibodies and inactivating undesired proteins permits the production IgG- or IgM-sensitized cells. The resultant cells have agglutination reaction strengths of about 2-4+. Erythrocytes from humans r other animals may undergo the monospecific sensitization. Other human proteins, such as IgA or C5, and plasma proteins from other animals may similarly be attached monospecifically to red blood cells.