Acylase catalysis in absence of heavy metals e.g. cobalt - for prodn. of L-amino acids by stereospecific hydrolysis of (N)-acyl-D,L-amino acids

摘要

In a new procedure for catalysers with acylase in the absence of heavy metals, (a) the acylase is treated with a complexing agent, (b) the complexing agent is removed, (c) a physiologically innocuous activating metal is added, and (d) the catalysis is carried out with the acylase activated with this metal. The complexing agent is EDTA. Pref. physiologically innocuous activating metals are Ca and Mg. The acylase catalysis is pref. carried out continuously at a metal ion concn. of 0.1-100 (pref. 1-70, esp. 5-50)mmol/l and the product concn. is continuously measured and the values obtd. used to control the metal ion concn. Acylases are used for the stereospecific hydrolysis of N-acyl derivs. of L-aminoacids in the prodn. of optically pure L-amino acids, which are used in the food and pharmaceutical industries. Contamination of L-amino acid products with undesirable heavy metals (e.g. cobalt) is avoided.