Increased depth of focus and resolution in light microscopes - images any object planes with powerful magnifications

摘要

The image of an object is scanned point-by-point by means of a single-or multi-hole scanning diaphragm, which lies in a plane conjugated to the plane onto which the objective is focussed. The scanning diaphragm also scans the surface area of the object by means of a light spot pattern. The scanning of the object image and/or the object takes place in a direction (X and/or Y direction) parallel to the focussed plane, alternatively vertical to the focussed plane (Z direction). Scanning in the Z direction is by means of a periodic displacements of the object and/or the objective.