CONTINUOUS CULTURING OF CHLORELLA UNDER LIGHT IRRADIATION

摘要

PURPOSE:To improve the yield of chlorella in the high-concentration continuous culturing of chlorella in the presence of light and oxygen, using acetic acid as a carbon source, by controlling the pH and the concentration of acetic acid in the culturing system disturbed by the metabolized acids produced in the system. CONSTITUTION:Chlorella (e.g. Chlorella bulgaris) is cultured continuously in a medium under the light irradiation of 1-10W/1 liter, and in the presence of air or O2, using acetic acid as a carbon source. The pH and the concentration of acetic acid are adjusted to 6.0-7.5 and =2500ppm, respectively, by controlling the volume of acetic acid supplied continuously to the system. When the metabolized acids such as glycolic acid are accumulated in the system, it is neutralized with an alkali to ensure the supply of the necessary amount of acetic acid. By this process, the high-concentration culturing of chlorella can be carried out under light irradiation for a long period, and a high-quality chlorella containing a large amount of chlorophyll can be produced continuously, in high yield.